Annex

Hantavirus testing
Either

1. The colony was tested with negative results for hantavirus by enzyme-linked immunosorbent assay (ELISA) or multiplex fluorescent immunoassay (MFI).

   Animals tested were at least eight weeks of age.

   The number of animals tested provided 99% confidence of detecting disease at 25% prevalence.

   Laboratory reports must be provided and include:

   1.1. The number of animals in the colony.

   1.2. The number of animals tested.

   1.3. The date of blood collection.

   OR

2. Sentinels were tested with negative results for hantavirus by enzyme-linked immunosorbent assay (ELISA) or multiplex fluorescent immunoassay (MFI).

   Sentinels of the same species, 8-12 weeks of age were placed with the colony and remained in contact for a minimum of 45 days but not more than 120 days prior to testing.

   An appropriate number of sentinels were tested to provide 99% confidence of detecting disease at 25% prevalence. Note: A few additional sentinels should be added to the colony to allow for incidental losses.
   Laboratory reports must be provided and include:

   2.1. The number of animals in the colony.

   2.2. The date of introduction of sentinels.

   2.3. The number of sentinels tested.

   2.4. The date of blood collection.