Annex

Note: Veterinary certificates are negotiated directly between relevant government authorities. In cases where a veterinary certificate has not been negotiated, this annex can be used as the basis for developing a suitable document.

Please attach a summary table stating the stallion identifications, testing dates for the stallions, the collection dates, the number of straws/containers from each collection and straw/container markings

Permit number: …………………………..

Name of consignee: ………………………….

Address of consignee: ………………………………………………………………….

………………………………………………………………………………………….

I, …………………………………(name), being an official government veterinarian of Switzerland, hereby certify that:

1. This certificate relates to equine semen collected from a stallion which was resident on the following collection centre and the “Approval of equine semen collection certificate” (certificate 1) for this centre is attached.
   Name and address of approved semen collection centre:…………………………………………..
   The collection centre is currently approved.
2. The stallion, identified as………………………………………, entered the collection centre on…………….. (date).
3. Semen for this consignment was collected from this stallion on the following dates:
   Collection date:……………………
   Collection date:……………………
   Collection date:……………………
4. During the three months immediately prior to the first collection of semen for this consignment, the stallion was continuously resident in one or more of the following countries: Switzerland, Austria, Belgium, Denmark, France, Finland, Germany, Greece, Republic of Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, United Kingdom.
   
   AND
   
   i. These countries have been free from African horse sickness, dourine, vesicular stomatitis and glanders for the two years before the collection for this consignment.
   
   OR
   
   ii. Germany had been free from African horse sickness, dourine and vesicular stomatitis for the two years before the collection of semen for this consignment.
   
   AND
   
   The stallion/s were resident on premises in Germany that have been free from glanders for the past two years and Germany has a surveillance programme in place demonstrating the absence of glanders in accordance with chapter 1.4 of the OIE Terrestrial animal health code.
5. Whilst on the collection centre, the donor stallion and any in contact horses were not known to be suffering from the following diseases:
   - Borna disease
   - contagious equine metritis
   - equine infectious anaemia
   - equine viral arteritis
   - equine viral abortion (equine herpes virus 1, including the neurologic syndrome)
   - equine coital exanthema, and
   - salmonellosis (Salmonella abortus equi).

6. The donor stallion was examined by an approved veterinarian* on each day of collection of semen for this consignment and was clinically healthy.

   *An approved veterinarian is a veterinarian authorised by the government veterinary administration to supervise the semen collection centre.

7. Disease testing
   Microbiological and serological testing were performed by laboratories approved by the veterinary administration of the exporting country. Laboratory reports for all testing are attached to this certificate.
8. Contagious equine metritis (CEM)
   Commencing at least seven days after entering the approved collection centre, the donor stallion and any teaser mares used, were tested, with negative results, for Taylorella equigenitalis by culture of swabs, collected on 3 occasions at intervals of not less than seven days, taken from:
   - donors: the penile sheath, the urethra and the urethral sinus
   - teaser mares (if teaser mares are used): the clitoral fossa including the clitoral sinuses, and on one occasion from the endometrium of each teaser mare whilst in oestrus
   Date of swabbing donor stallion:.…………………….
   Date of swabbing donor stallion:.…………………….
   Date of swabbing donor stallion:.…………………….
   Dates of swabbing teaser mares (if teaser mares used):
   Date 1:……………..
   Date 2:………………
   Date 3:……………….
9. Equine infectious anaemia (EIA)
   At least 21 days after entering the approved equine semen collection centre, the stallion was tested by one of the following test methods with negative results.
   An approved enzyme-linked immunosorbent assay (ELISA)
   OR
   A Coggin’s test (AGID)
   Type of test:………………….. Date blood drawn:………………………
10. Equine viral arteritis (EVA)
    At least 21 days after entering the approved equine semen collection centre, the stallion was tested by one of the following methods with negative results.
    A serum neutralisation test (SNT) for EVA.
    Date of blood drawn…………………….
    OR
    A virus isolation test (VI) on two samples of semen.
    Date of semen collection:…………………………
    Date of semen collection:…………………………
    OR
    The stallion was test mated to two mares which showed negative results to SNT for EVA on two blood samples; one collected at the time of test mating (a) and another 28 days after the test mating (b).
    Dates of test mating with mare 1:……………………..
    Date of blood draw (a):……………………..
    Date of blood draw (b):…………………………
    
    Dates of test mating with mare 2:……………………..
    Date of blood draw (a):……………………..
    Date of blood draw (b):…………………………
11. Semen processing
    New, disposable artificial vagina (AV) liners and collection vessels were used for each collection.
    All biological products of animal origin used in the collection, processing and storage of semen were obtained from animals free from African horse sickness and vesicular stomatitis or sterilised by approved methods.
    Sanitary precautions were observed during collection and processing of the semen to prevent contamination.
    Approved antibiotics were added to the semen extender/diluent in generally accepted levels.
12. Sex sorted semen
    For sex sorted semen, either:
    - Sex sorted semen is NOT included in this shipment,
    OR
    - Sex sorted semen IS included in this shipment, and:
    - equipment used for sex-sorting sperm was cleaned and disinfected between animals according the sex semen licensor’s recommendations; and
    - where seminal plasma, or components thereof, was added to sorted semen prior to cryopreservation and storage, it was derived from animals of same or better health status.
    [One of the options must be struck out.]
13. Storage and transport– unfrozen (fresh, chilled) semen
    The semen for export, all of equivalent health status, was identified and placed under the supervision of the official government veterinarian in chilled transport container(s) (such as Equitainers) which was either: (one of the options below must be struck out)
    - new, or
    - disinfected on ………………………. (date), using the active ingredient: ……………………
    Note: The following disinfectants will be accepted by the Department of Agriculture, Fisheries and Forestry: 2% available chlorine (eg. chlorine bleach), 2% Virkon, or irradiation at 50 kGy.
    The semen was stored under security and under my supervision until export.
14. Storage and transport – frozen semen
    The semen for export, all of equivalent health status, was identified and placed under the supervision of the official government veterinarian in new liquid nitrogen in a container(s) which was either: (one of the options below must be struck out)
    - new, or
    - prior to loading, the shipper was emptied and inspected and any loose straws removed. The shipper, including all surfaces contacting the straws, was disinfected on:…………………………….…..(dated), using the active ingredient:……………………
    Note: The following disinfectants will be accepted by the Department of Agriculture, Fisheries and Forestry: 2% available chlorine (eg chlorine bleach), 2% Virkon, or irradiation at 50kGy.
15. Storage - fresh and frozen semen
    Reproductive material for export to Australia was identified in a legible and non-erasable manner, and was stored since the end of the collection period until export under the supervision of the government approved veterinarian(s) in containers in which no biological material other than semen, embryos or ova of equivalent health status as specified in this veterinary certificate was held.
16. For this reproductive material, either:
    - Reproductive material was NOT removed from containers for further processing or aggregation with other reproductive material.
    or
    - Reproductive material was removed from containers for further processing or aggregation with other reproductive material at an approved centre or laboratory. The dates of transfer, reason for transfer (e.g. for sex sorting), name of the approved centre or laboratory and the approved veterinarian ARE listed against the containers. The unique serial (SEAL) number of each shipping container IS included in this documentation.
    Date of transfer...................................
    Reason for transfer ..................................................................................................................
    Name of approved laboratory...................................................................................................
    Approved veterinarian/s...........................................................................................................
    Container serial number/s.........................................................................................................
    [One of the options must be struck out.]
17. Disease testing, semen collection and semen processing and storage were supervised by an official government veterinarian.
18. The transport container was sealed with an official government seal prior to export.

Seal number: ………………………..