Annex

Spring viraemia of carp virus (SVCV): This certification must be based on negative test results on 3 batches of testing within the 6 month period immediately prior to export. The fish population from which the goldfish originate must be tested for SVCV using virus isolation and identification methods (virus neutralisation VN, immunofluorescence or enzyme-linked immunosorbent essay (ELISA), as described in the OIE 'Diagnostic Manual for Aquatic Animal Diseases'. Certification must be based on a minimum testing of 3 batches within the 6 month period immediately prior to export. The number of fish sampled must ensure a 95 % confidence level of disease agent/pest detection in a source fish population with an agent prevalence of 2%. The population size is defined as the number of fish of the same species that share a common water supply and originate from the same broodstock.

Aeromonas salmonicida: This certification must be based on negative test results on 3 batches of testing within the 6 month period immediately prior to export. The fish in the population from which the goldfish originate must be tested for A. salmonicida using standard bacteriological methods. Tissues tested should include skin lesions (if present), kidney, liver and spleen. Testing must be based on internationally accepted methods of isolation, culture and identification. Certification must be based on a minimum testing of 3 batches within the 6 month period immediately prior to export. The number of fish sampled must ensure a 95 % confidence level of disease agent/pest detection in a source fish population with an agent prevalence of 2%. The population size is defined as the number of fish of the same species that share a common water supply and originate from the same broodstock.

Certification of freedom from specified disease agents or pests (that is, SVCV, A. salmonicida, Dactylogyrus vastator and D. extensus) apply to the country, zone or export premises from which fish are exported to Australia. If the fish originated from a country other than the exporting country within 6 months of export, certification attesting to the health of the source fish population must accompany all shipments.

Countries, zones or premises declared free or in the process of establishing freedom from specified disease agents or pests, must take adequate precautions to prevent introduction of the disease agent or pest into the country, zone or premises, including via infected fish or water.

After two years of surveillance with laboratory tests and in the absence of suspect clinical signs or suspect positive test results, twice yearly testing must continue but the sample size at each inspection may be reduced to 30 fish of the species being exported (including broodfish when available), provided that all introductions of new fish to the population are sourced from fish populations that meet equivalent criteria or superior health standard.